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This study aimed to develop one-tube colorimetric reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay by incorporating coronavirus RNA purification, amplification and detection in a single tube for easy detection of SARS-CoV-2 RNA.
RT-LAMP is a cheap,
rapid, effective, and highly sensitive diagnostic assay for COVID-19 RNA
detection. For COVID-19 patients, it can be potentially used for routine
screening and point of care testing.
This study aimed to
develop one-tube colorimetric reverse transcription-loop-mediated isothermal
amplification (RT-LAMP) assay by incorporating coronavirus RNA purification,
amplification and detection in a single tube for easy detection of SARS-CoV-2
RNA.
A combination of silanol-rich magnetic beads-based rapid RNA extraction and quick isothermal amplification in a single tube, bypasses the RNA elution phase and directly amplifies the on-beads RNA molecules with the visualized outcomes.
This assay has a limit of detection as low as
200 copies per mL for sample input volumes of approximately 600 μL. It can be
executed in <1 hour from sample collection till the display of the result.
This assay had a high specificity and a 100%
concordance with the standard test, i.e. RT-qPCR test after the evaluation of
29 clinical samples.
For visual detection
of SARS-CoV-2 RNA, RT-LAMP assay serves as a quick and highly sensitive
diagnostic test and is specifically appropriate for usage at township hospitals
or community clinics.
Biosensors & Bioelectronics
Rapid and highly sensitive one-tube colorimetric RT-LAMP assay for visual detection of SARS-CoV-2 RNA
Yugan He et al.
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