Blood samples were obtained in Sodium Citrate (SC) tubes, Serum Separator Tubes (SST tubes), and Acid Citrate Dextrose (ACD) tubes and uricase method was used to measure the circulating UA concentrations. Impact of dextrose, citric acid and sodium citrate in the presence of peroxidase, violet dye, and hydrogen peroxide determined by absorbance assays.

As compared to the SST tubes, UA concentrations collected in ACD and SC tubes were found to be lower. Further, different concentration of citric acid, dextrose and sodium citrate demonstrated significantly lower UA measurements than controls. In the presence of leuco crystal violet, hydrogen peroxide, and peroxidase, absorbance assays showed that increased concentrations of citric acid and sodium citrate associated with the reduced amount of oxidized dye in the uricase method of UA measurement in a dose-dependent manner. The reason behind this was the scavenging of hydrogen peroxide by citric acid, and this reduces the quantity of oxidized leuco dye which leads to false low UA measurements. However, dextrose was not associated with the altered amount of oxidized dye. Therefore, the ACD and SC tubes should not be used while measuring the UA through uricase measurement methods.